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The reconstitution was accomplished by incubation with a reaction medium containing the protein, via its small amphiphatic α-helix [80]. Ferricinium methanol was used as an artificial electron acceptor and the apparent Michaelis Menten constants of Pox both for pyruvate and ferricium were estimated, in fair agreement to what can be deduced from the literature [81]. M. Hawkridge and co-workers are attributed to studies of cytochrome c oxidase incorporated in an HBM. The biomimetic membrane is formed by immersing a thiolated gold surface in a buffer solution of deoxycholate, phospholipids and cytochrome c oxidase.

Hydrophilic amino groups were linked to the polymer and a phospholipid bilayer was transferred to the ca. 80 Å thick hydrated polymer film. The first layer was obtained with Langmuir-Blodgett techniques and the second leaflet with Langmuir-Schäfer techniques. The fluidity of the lipids was investigated with photobleaching techniques and it was found that the lipid bilayer was fluid and stable for several days. A hydrophilic polymer cushion with a smooth outer surface, is easily self-assembled at the electrode surface by alternate adsorption of polycations and polyanions [ 1 15].

Ringsdorf, H. and Sackmann, E. (1996) Influence of anchor lipids on the homogeneity and mobility of lipid bilayers on thin polymer films, Angew. Chem. Int. Ed. Engl. 35, 1682-1685. 29. Kalb, E. K. (1992) Incorporation of cytochrome b 5 into supported phospholipid bilayers by vesicle fusion to supported monolayers, Thin Solid Films 210/211, 763-765. 30. , Offenhausser, A. and Knoll, W. (1999) The peptide-tethered lipid membrane as a biomimetic system to incorporate cytochrome c oxidase in a functionally active form, Biosens.

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An interferometer method of observing the vibrations of an oscillating quartz plate by Osterberg H.


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